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Clinical relevance of biotechnology advances
/content/chapter/10.22233/9781905319541.chap21
Clinical relevance of biotechnology advances
- Author: Gaia Cecilia Luvoni
- From: BSAVA Manual of Canine and Feline Reproduction and Neonatology
- Item: Chapter 21, pp 212 - 221
- DOI: 10.22233/9781905319541.21
- Copyright: © 2010 British Small Animal Veterinary Association
- Publication Date: January 2010
Abstract
Biotechnology offers new strategies to improve the treatment of male and female infertility and to preserve biodiversity. This chapter looks at in vivo sperm-oocyte interaction: fertilization; in vitro sperm-oocyte interaction: in vitro embryo production; in vitro sperm-oocyte interaction: in vitro sperm function tests; cloning.
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Figures
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21.1
(a) Immature canine oocyte at the germinal vesicle (GV) stage of meiosis and (b) stained with fluorescent dye (Hoechst 33342) to visualize the GV nucleus. (c) Mature feline oocyte at the metaphase II (MII) stage of meiosis and (d) stained with fluorescent dye (Hoechst 33342) to visualize the MII plate and the first polar body. © 2010 British Small Animal Veterinary Association
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21.1
(a) Immature canine oocyte at the germinal vesicle (GV) stage of meiosis and (b) stained with fluorescent dye (Hoechst 33342) to visualize the GV nucleus. (c) Mature feline oocyte at the metaphase II (MII) stage of meiosis and (d) stained with fluorescent dye (Hoechst 33342) to visualize the MII plate and the first polar body.
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21.2
The acrosome reaction. (a) Spermatic head with intact acrosome. (b) Fusion and vesiculation of the plasma membrane and outer acrosomal membrane with release of hydrolytic enzymes. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
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21.2
The acrosome reaction. (a) Spermatic head with intact acrosome. (b) Fusion and vesiculation of the plasma membrane and outer acrosomal membrane with release of hydrolytic enzymes. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.3
(a) Immature canine oocyte (grade 1). (b) Immature feline oocyte (grade 1). © 2010 British Small Animal Veterinary Association
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21.3
(a) Immature canine oocyte (grade 1). (b) Immature feline oocyte (grade 1).
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21.4
In vitro fertilization procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
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21.4
In vitro fertilization procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.5
Feline embryos obtained by IVF. © 2010 British Small Animal Veterinary Association
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21.5
Feline embryos obtained by IVF.
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21.6
ICSI procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
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21.6
ICSI procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.7
In vitro tests of sperm function. 1 = acrosome intact spermatozoon. 2 = zona pellucida binding. 3 = zona pellucida penetration. 4 = oocyte penetration. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
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21.7
In vitro tests of sperm function. 1 = acrosome intact spermatozoon. 2 = zona pellucida binding. 3 = zona pellucida penetration. 4 = oocyte penetration. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.8
Zona binding assay: canine oocyte stained with fluorescent dye (Hoechst 33342) to visualize spermatozoa bound to the zona pellucida. © 2010 British Small Animal Veterinary Association
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21.8
Zona binding assay: canine oocyte stained with fluorescent dye (Hoechst 33342) to visualize spermatozoa bound to the zona pellucida.
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21.9
Cloning procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
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21.9
Cloning procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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