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Clinical relevance of biotechnology advances

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Abstract

Biotechnology offers new strategies to improve the treatment of male and female infertility and to preserve biodiversity. This chapter looks at sperm-oocyte interaction: fertilization; sperm-oocyte interaction: embryo production; sperm-oocyte interaction: sperm function tests; cloning.

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/content/chapter/10.22233/9781905319541.chap21

Figures

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21.1 Immature canine oocyte at the germinal vesicle (GV) stage of meiosis and stained with fluorescent dye (Hoechst 33342) to visualize the GV nucleus. Mature feline oocyte at the metaphase II (MII) stage of meiosis and stained with fluorescent dye (Hoechst 33342) to visualize the MII plate and the first polar body.
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21.2 The acrosome reaction. Spermatic head with intact acrosome. Fusion and vesiculation of the plasma membrane and outer acrosomal membrane with release of hydrolytic enzymes. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.3 Immature canine oocyte (grade 1). Immature feline oocyte (grade 1).
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21.4 fertilization procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.5 Feline embryos obtained by IVF.
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21.6 ICSI procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.7 tests of sperm function. 1 = acrosome intact spermatozoon. 2 = zona pellucida binding. 3 = zona pellucida penetration. 4 = oocyte penetration. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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21.8 Zona binding assay: canine oocyte stained with fluorescent dye (Hoechst 33342) to visualize spermatozoa bound to the zona pellucida.
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21.9 Cloning procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.

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