Full text loading...
Clinical relevance of biotechnology advances
/content/chapter/10.22233/9781905319541.chap21
Clinical relevance of biotechnology advances
- Author: Gaia Cecilia Luvoni
- From: BSAVA Manual of Canine and Feline Reproduction and Neonatology
- Item: Chapter 21, pp 212 - 221
- DOI: 10.22233/9781905319541.21
- Copyright: © 2010 British Small Animal Veterinary Association
- Publication Date: January 2010
Abstract
Biotechnology offers new strategies to improve the treatment of male and female infertility and to preserve biodiversity. This chapter looks at in vivo sperm-oocyte interaction: fertilization; in vitro sperm-oocyte interaction: in vitro embryo production; in vitro sperm-oocyte interaction: in vitro sperm function tests; cloning.
Preview this chapter:
Clinical relevance of biotechnology advances, Page 1 of 1
< Previous page | Next page > /docserver/preview/fulltext/10.22233/9781905319541/9781905319541.21-1.gif
/content/chapter/10.22233/9781905319541.chap21
Figures
/content/figure/10.22233/9781905319541.chap21.ch21fig1
21.1
(a) Immature canine oocyte at the germinal vesicle (GV) stage of meiosis and (b) stained with fluorescent dye (Hoechst 33342) to visualize the GV nucleus. (c) Mature feline oocyte at the metaphase II (MII) stage of meiosis and (d) stained with fluorescent dye (Hoechst 33342) to visualize the MII plate and the first polar body. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_1_thumb.gif
10.22233/9781905319541/fig21_1.png
21.1
(a) Immature canine oocyte at the germinal vesicle (GV) stage of meiosis and (b) stained with fluorescent dye (Hoechst 33342) to visualize the GV nucleus. (c) Mature feline oocyte at the metaphase II (MII) stage of meiosis and (d) stained with fluorescent dye (Hoechst 33342) to visualize the MII plate and the first polar body.
/content/figure/10.22233/9781905319541.chap21.ch21fig2
21.2
The acrosome reaction. (a) Spermatic head with intact acrosome. (b) Fusion and vesiculation of the plasma membrane and outer acrosomal membrane with release of hydrolytic enzymes. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_2_thumb.gif
10.22233/9781905319541/fig21_2.png
21.2
The acrosome reaction. (a) Spermatic head with intact acrosome. (b) Fusion and vesiculation of the plasma membrane and outer acrosomal membrane with release of hydrolytic enzymes. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
/content/figure/10.22233/9781905319541.chap21.ch21fig3
21.3
(a) Immature canine oocyte (grade 1). (b) Immature feline oocyte (grade 1). © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_3_thumb.gif
10.22233/9781905319541/fig21_3.png
21.3
(a) Immature canine oocyte (grade 1). (b) Immature feline oocyte (grade 1).
/content/figure/10.22233/9781905319541.chap21.ch21fig4
21.4
In vitro fertilization procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_4_thumb.gif
10.22233/9781905319541/fig21_4.png
21.4
In vitro fertilization procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
/content/figure/10.22233/9781905319541.chap21.ch21fig5
21.5
Feline embryos obtained by IVF. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_5_thumb.gif
10.22233/9781905319541/fig21_5.png
21.5
Feline embryos obtained by IVF.
/content/figure/10.22233/9781905319541.chap21.ch21fig6
21.6
ICSI procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_6_thumb.gif
10.22233/9781905319541/fig21_6.png
21.6
ICSI procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
/content/figure/10.22233/9781905319541.chap21.ch21fig7
21.7
In vitro tests of sperm function. 1 = acrosome intact spermatozoon. 2 = zona pellucida binding. 3 = zona pellucida penetration. 4 = oocyte penetration. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_7_thumb.gif
10.22233/9781905319541/fig21_7.png
21.7
In vitro tests of sperm function. 1 = acrosome intact spermatozoon. 2 = zona pellucida binding. 3 = zona pellucida penetration. 4 = oocyte penetration. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
/content/figure/10.22233/9781905319541.chap21.ch21fig8
21.8
Zona binding assay: canine oocyte stained with fluorescent dye (Hoechst 33342) to visualize spermatozoa bound to the zona pellucida. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_8_thumb.gif
10.22233/9781905319541/fig21_8.png
21.8
Zona binding assay: canine oocyte stained with fluorescent dye (Hoechst 33342) to visualize spermatozoa bound to the zona pellucida.
/content/figure/10.22233/9781905319541.chap21.ch21fig9
21.9
Cloning procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission. © 2010 British Small Animal Veterinary Association
10.22233/9781905319541/fig21_9_thumb.gif
10.22233/9781905319541/fig21_9.png
21.9
Cloning procedure. Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.