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Diagnostic procedures

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This chapter covers the adaptation of familiar techniques of sample collection for guinea pigs and explains the anatomical and behavioural peculiarities in the guinea pig that may alter the approach to preparation of patients and choice of equipment. Step-by-step guides to the collection of blood, urine, respiratory tract, ocular, dermatological and lymph node samples are followed by a comprehensive guide to choosing the appropriate diagnostic procedure.

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Figures

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5.1 Veins suitable for venepuncture in the guinea pig. (a) Saphenous (arrowed) and metatarsal (arrowhead) veins. (b) Cephalic vein (arrowed).
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5.3 Needle hub with capillary tube prepared for blood sampling.
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5.4 (a) Cranial vena cava anatomy in the guinea pig. (b) The approach to alternative vessels for blood sampling, with the animal in dorsal recumbency. Needle position 1 shows sampling from the right jugular vein; needle position 2 shows sampling from the vena cava, as approached from the animal’s right hand-side, with the needle held at 30 degrees to horizontal and directed towards the opposite hip; needle position 3 shows sampling from the vena cava, as approached from the animal’s left-hand side, with the needle held at 30 degrees to horizontal and directed towards the opposite hip. (a, Reproduced from the , © Vittorio Capello) Drawn by S.J. Elmhurst BA Hons (www.livingart.org.uk) and reproduced with her permission.
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5.6 Excisional biopsy of a melanoma from the lower eyelid of a Bryant guinea pig. (a) Before removal of the lesion. (b) After removal.
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Video 5.1 Skin scraping technique.
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5.8 (a, b) Technique for taking scrapings from a skin lesion.
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5.9 Microscopic appearance of a mite (original magnification X40).
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5.10 (a) Material removed from a follicular cyst. (b) Aspiration of an intradermal mass on the left paralumbar fossa, using a 22 G needle.

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Skin scraping technique

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