Adrenal gland imaging is performed as part of the assessment of a patient when there is suspicion of abnormal adrenal gland function. Information that is of interest to a veterinary surgeon includes the size, shape and location of both glands, the internal structure, the presence of any local invasion into adjacent vessels or organs, and whether there is any evidence of distant metastases. Adrenal gland imaging alone cannot be used as an indicator of adrenal gland function, and findings should be interpreted in the light of the clinical presentation and results of other diagnostic tests. The chapter covers Overview of anatomy; Radiography; Ultrasonography; Overview of additional imaging modalities; and Adrenal gland diseases.