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Core investigative and laboratory techniques
/content/chapter/10.22233/9781905319886.chap3
Core investigative and laboratory techniques
- Author: Janet D. Littlewood
- From: BSAVA Manual of Canine and Feline Dermatology
- Item: Chapter 3, pp 24 - 30
- DOI: 10.22233/9781905319886.3
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Copyright:
- Publication Date: September 2012
Abstract
PLEASE NOTE A NEW 4TH EDITION HAS BEEN PUBLISHED AND IS AVAILABLE IN THE LIBRARY AND IN PRINT
Whilst a complete history and thorough clinical examination are essential for dermatological cases, further diagnostic investigations are of paramount importance in reaching a definitive diagnosis. Many can be performed as part of, or at the conclusion of, the clinical examination. Any samples sent to the laboratory are subject to local and national postal regulations. The following are considered: Equipment; Coat brushings; Samples for direct microscopy; Culture of microorganisms; Skin biopsy; and Faecal examination.
/content/chapter/10.22233/9781905319886.chap3
Figures
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3.1
Hair pluck to show primary (P) and secondary (S) hairs. The blunt tips and frayed tips to several hairs indicate self-inflicted damage. © 2012 British Small Animal Veterinary Association
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3.1
Hair pluck to show primary (P) and secondary (S) hairs. The blunt tips and frayed tips to several hairs indicate self-inflicted damage.
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3.2
Dermatophytosis. A hair shaft infected with Trichophyton spp., showing disruption of the structure due to invasion of fungal elements, with ectothrix spores surrounding the damaged hair. © 2012 British Small Animal Veterinary Association
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3.2
Dermatophytosis. A hair shaft infected with Trichophyton spp., showing disruption of the structure due to invasion of fungal elements, with ectothrix spores surrounding the damaged hair.
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3.3
Telogen hair roots. © 2012 British Small Animal Veterinary Association
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3.3
Telogen hair roots.
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3.4
Adhesive tape detection of parasites. (a)
Archeopsylla erinacei, the hedgehog flea, found on a terrier. (b) Close up of the head and anterior thorax showing two short pronotal combs (black arrows) and the slanted row of short genal combs (white arrow). (c)
Spilopsyllus cuniculi, the stick-tight flea of rabbits, found on the pinna of a cat. (d)
Trichodectes canis, the biting or chewing louse. (e)
Lignognathus setosus, the sucking louse. (f)
Cheyletiella yasguri mites and eggs collected from the dorsum of a Boxer. (g) High-power magnification of Cheyletiella parasitovorax to show hooked mouthparts and globoid sense organ. (Courtesy of Kansas State University) © 2012 British Small Animal Veterinary Association
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3.4
Adhesive tape detection of parasites. (a)
Archeopsylla erinacei, the hedgehog flea, found on a terrier. (b) Close up of the head and anterior thorax showing two short pronotal combs (black arrows) and the slanted row of short genal combs (white arrow). (c)
Spilopsyllus cuniculi, the stick-tight flea of rabbits, found on the pinna of a cat. (d)
Trichodectes canis, the biting or chewing louse. (e)
Lignognathus setosus, the sucking louse. (f)
Cheyletiella yasguri mites and eggs collected from the dorsum of a Boxer. (g) High-power magnification of Cheyletiella parasitovorax to show hooked mouthparts and globoid sense organ. (Courtesy of Kansas State University)
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3.5
Adhesive tape strip with detail of squames with many yeast organisms in a case of Malassezia pedal dermatitis. © 2012 British Small Animal Veterinary Association
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3.5
Adhesive tape strip with detail of squames with many yeast organisms in a case of Malassezia pedal dermatitis.
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3.6
Demodex canis mites and cellular debris in a liquid paraffin-mounted preparation of a follicular smear. (Courtesy of Kansas State University) © 2012 British Small Animal Veterinary Association
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3.6
Demodex canis mites and cellular debris in a liquid paraffin-mounted preparation of a follicular smear. (Courtesy of Kansas State University)
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3.7
Sampling site for skin scrapes from a dog with suspected sarcoptic mange. Hair has been clipped over the lateral elbow to show early lesions of crusted papular eruptions. © 2012 British Small Animal Veterinary Association
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3.7
Sampling site for skin scrapes from a dog with suspected sarcoptic mange. Hair has been clipped over the lateral elbow to show early lesions of crusted papular eruptions.
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3.8
(a)
Sarcoptes scabei mite, adult female. (Courtesy of Kansas State University) (b) Group of Sarcoptes scabei mite eggs from an epidermal burrow. © 2012 British Small Animal Veterinary Association
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3.8
(a)
Sarcoptes scabei mite, adult female. (Courtesy of Kansas State University) (b) Group of Sarcoptes scabei mite eggs from an epidermal burrow.
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3.9
(a,b) Pustule cytology, stained with Diff-Quik®, showing neutrophils with intracellular cocci (arrowed) in a case of staphylococcal pyoderma. (c) Cytology of an aural exudate showing bacteria, predominantly rods (arrowed), and inflammatory cells with chromatin streaks. © 2012 British Small Animal Veterinary Association
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3.9
(a,b) Pustule cytology, stained with Diff-Quik®, showing neutrophils with intracellular cocci (arrowed) in a case of staphylococcal pyoderma. (c) Cytology of an aural exudate showing bacteria, predominantly rods (arrowed), and inflammatory cells with chromatin streaks.
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3.10
Fine-needle aspirate from a mass, showing epithelial cells (large cells with central nucleus; arrowed) and cellular debris. The mass was later confirmed as a sebaceous adenoma. © 2012 British Small Animal Veterinary Association
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3.10
Fine-needle aspirate from a mass, showing epithelial cells (large cells with central nucleus; arrowed) and cellular debris. The mass was later confirmed as a sebaceous adenoma.
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3.11
Dermatophyte test medium: colour change from yellow to red 5 days after inoculation, indicating growth of a dermatophyte. © 2012 British Small Animal Veterinary Association
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3.11
Dermatophyte test medium: colour change from yellow to red 5 days after inoculation, indicating growth of a dermatophyte.