Full text loading...
Practical laboratory techniques
/content/chapter/10.22233/9781910443064.chap10
Practical laboratory techniques
- Author: Clare Knottenbelt
- From: BSAVA Manual of Practical Veterinary Nursing
- Item: Chapter 10, pp 205 - 228
- DOI: 10.22233/9781910443064.10
- Copyright: © 2007 British Small Animal Veterinary Association
- Publication Date: January 2007
Abstract
This chapter is designed to give information on health and safety in the practice laboratory, the maintenance and use of laboratory equipment, the Collection, preparation and preservation of samples, and techniques for the laboratory tests commonly performed in veterinary practice. This includes blood samples; urine; faeces; body fluids; fine needle aspiration and skin and hair sampling.
Preview this chapter:
Practical laboratory techniques, Page 1 of 1
< Previous page | Next page > /docserver/preview/fulltext/10.22233/9781910443064/9781910443064.10-1.gif
/content/chapter/10.22233/9781910443064.chap10
Figures
/content/figure/10.22233/9781910443064.chap10.ch10ufig1
Untitled
© 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_4_thumb.gif
10.22233/9781910443064/fig10_4.png
/content/figure/10.22233/9781910443064.chap10.ch10fig9
10.9
Microhaematocrit centrifuge. Tubes are placed on opposite sides of the centrifuge to ensure that the drum is balanced during spinning. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_9_thumb.gif
10.22233/9781910443064/fig10_9.png
10.9
Microhaematocrit centrifuge. Tubes are placed on opposite sides of the centrifuge to ensure that the drum is balanced during spinning.
/content/figure/10.22233/9781910443064.chap10.ch10fig10
10.10
Appearance of a capillary tube after centrifugation. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_10_thumb.gif
10.22233/9781910443064/fig10_10.png
10.10
Appearance of a capillary tube after centrifugation.
/content/figure/10.22233/9781910443064.chap10.ch10fig13
10.13
Preparing a blood smear by the draw back and push away method. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_13_thumb.gif
10.22233/9781910443064/fig10_13.png
10.13
Preparing a blood smear by the draw back and push away method.
/content/figure/10.22233/9781910443064.chap10.ch10fig14
10.14
Examples of good and bad blood smears. (Adapted from BSAVA Manual of Canine and Feline Clinical Pathology, 2nd edition) © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_14_thumb.gif
10.22233/9781910443064/fig10_14.png
10.14
Examples of good and bad blood smears. (Adapted from BSAVA Manual of Canine and Feline Clinical Pathology, 2nd edition)
/content/figure/10.22233/9781910443064.chap10.ch10fig18
10.18
Haemocytometer grid. To perform a white cell count, the numbers of white cells in the squares marked W, X, Y and Z are totalled and multiplied by 50. When a red cell count is being performed the squares marked A, B, C, D and E are totalled and divided by 100. Note: When performing a red cell count, dilute blood to 1 in 200 with 3% sodium citrate and 40% formol saline. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_18_thumb.gif
10.22233/9781910443064/fig10_18.png
10.18
Haemocytometer grid. To perform a white cell count, the numbers of white cells in the squares marked W, X, Y and Z are totalled and multiplied by 50. When a red cell count is being performed the squares marked A, B, C, D and E are totalled and divided by 100. Note: When performing a red cell count, dilute blood to 1 in 200 with 3% sodium citrate and 40% formol saline.
/content/figure/10.22233/9781910443064.chap10.ch10fig19
10.19
Appearance of the common white blood cells following staining with Leishman’s. (a) Canine eosinophil and neutrophil. Note the granular appearance in the cytoplasm of the eosinophil. Both cells have a segmented nucleus. (b) Canine lymphocyte. Note the large rounded nucleus with little cytoplasm. (c) Feline neutrophils. Note the segmented nuclei. Some of these neutrophils are giant neutrophils, which are produced in association with severe inflammation. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_19_thumb.gif
10.22233/9781910443064/fig10_19.png
10.19
Appearance of the common white blood cells following staining with Leishman’s. (a) Canine eosinophil and neutrophil. Note the granular appearance in the cytoplasm of the eosinophil. Both cells have a segmented nucleus. (b) Canine lymphocyte. Note the large rounded nucleus with little cytoplasm. (c) Feline neutrophils. Note the segmented nuclei. Some of these neutrophils are giant neutrophils, which are produced in association with severe inflammation.
/content/figure/10.22233/9781910443064.chap10.ch10fig20
10.20
(a) Feline blood smear stained with Diff-Quik. Note the appearance of the neutrophils (N) and platelets (P). Platelets can sometimes be hard to see; if this is a problem, the slide should be dipped into the final stain (purple) seven times instead of five as this increases platelet staining. (b) Blood smear from a bird. The red cells are nucleated, which makes it difficult for automated analysers to differentiate them from white blood cells. (b, Courtesy of John Chitty) © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_20_thumb.gif
10.22233/9781910443064/fig10_20.png
10.20
(a) Feline blood smear stained with Diff-Quik. Note the appearance of the neutrophils (N) and platelets (P). Platelets can sometimes be hard to see; if this is a problem, the slide should be dipped into the final stain (purple) seven times instead of five as this increases platelet staining. (b) Blood smear from a bird. The red cells are nucleated, which makes it difficult for automated analysers to differentiate them from white blood cells. (b, Courtesy of John Chitty)
/content/figure/10.22233/9781910443064.chap10.ch10tab24
10.24
Summary of the tests used in routine urinalysis. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_24_thumb.gif
10.22233/9781910443064/fig10_24.png
10.24
Summary of the tests used in routine urinalysis.
/content/figure/10.22233/9781910443064.chap10.ch10fig26
10.26
Microscopic appearance of some of the common urinary crystals. (a) Struvite (triple phosphate) crystals. (b) Calcium oxalate crystals. (c) Calcium carbonate crystals. (d) Ammonium urate crystals. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_26_thumb.gif
10.22233/9781910443064/fig10_26.png
10.26
Microscopic appearance of some of the common urinary crystals. (a) Struvite (triple phosphate) crystals. (b) Calcium oxalate crystals. (c) Calcium carbonate crystals. (d) Ammonium urate crystals.
/content/figure/10.22233/9781910443064.chap10.ch10fig29
10.29
Identification of the common intestinal parasites of dogs and cats. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_29_thumb.gif
10.22233/9781910443064/fig10_29.png
10.29
Identification of the common intestinal parasites of dogs and cats.
/content/figure/10.22233/9781910443064.chap10.ch10fig37
10.37
Appearance of the common mites of the dog and cat. (a)
Otodectes. (b)
Sarcoptes adult. (c)
Sarcoptes eggs (high magnification). (d)
Demodex.
(e)
Cheyletiella yasguri mites and eggs collected from the dorsum of a Boxer. (a–d, © A. Foster/S. Shaw, University of Bristol; e, reproduced from BSAVA Manual of Small Animal Dermatology, 2nd edn.) © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_37_thumb.gif
10.22233/9781910443064/fig10_37.png
10.37
Appearance of the common mites of the dog and cat. (a)
Otodectes. (b)
Sarcoptes adult. (c)
Sarcoptes eggs (high magnification). (d)
Demodex.
(e)
Cheyletiella yasguri mites and eggs collected from the dorsum of a Boxer. (a–d, © A. Foster/S. Shaw, University of Bristol; e, reproduced from BSAVA Manual of Small Animal Dermatology, 2nd edn.)
/content/figure/10.22233/9781910443064.chap10.ch10fig41
10.41
Inoculating an agar plate using the streaking method. The loop should be flamed and cooled between each ‘streaking’. © 2007 British Small Animal Veterinary Association
10.22233/9781910443064/fig10_41_thumb.gif
10.22233/9781910443064/fig10_41.png
10.41
Inoculating an agar plate using the streaking method. The loop should be flamed and cooled between each ‘streaking’.