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Clinical pathology and post-mortem examination

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Abstract

When a live bird is presented for euthanasia and post-mortem examination it is a good habit to collect as much blood as possible from the right jugular vein: this will provide haematological and biochemical values in addition to the necropsy findings. This chapter evaluates haematology, cytology, microscopic evaluation, microbiological culture, diagnostic tests for infectious diseases, tests for specific conditions in raptors and post-mortem examination.

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Figures

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9.4 Blood cell morphology: red blood cells; heterophil; lymphocyte; monocyte; eosinophil; thrombocytes. (Diff-Quik, original magnification X1000) (© John Chitty)
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9.5 Gouldian finch with yeast-related enteropathy: wet mount; stained with Hemacolor. Arrows point to bacteria; arrowheads point to yeasts. (Original magnification: (a) X40; (b) X100)
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9.6 Growth of ) on blood agar and selective brilliant green phenol agar.
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9.7 conidia on conidiophores in wet mount; growth on malt agar after 48 hours.
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9.8 Enlarged bursa of Fabricius (arrowed) in a Canary with polyomavirus. (Courtesy of Peter Coutteel)
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9.10 Lay-out material and instruments for a PME: (1) two scalpel blades and handles; (2) one pair anatomical and one pair surgical forceps and two pair scissors; (3) bone-cutting forceps; (4) containers with 10% buffered formalin; (5) saline with pipette for wet mounts; (6) clean marked glass slides for cytology and wet mounts (parasitology); (7) burner for heating and sterilizing a scalpel blade and wire loop for culturing; (8) culture media for bacteriology and mycology; (9) wire loops for microbiological sampling; (10) container with disinfectant for the instruments; (11) aluminium foil plates for holding small organs or tissues; (12) waterproof marker; (13) preprinted labels; (14) copy of PME work-form and checklist; (15) paper towels for cleaning in between; (16) ruler.
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9.15 Haemorrhages in the gizzard of a falcon.
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9.16 Amyloidosis and hydrops ascites in a Gyrfalcon × Lanner falcon: liver amyloidosis and hydrops . spleen amyloidosis; lungs with severe oedema kidney amyloidosis.
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9.17 Mycotic air sacculitis due to aspergillosis in a Capuchinbird. Subacute air sac aspergillosis in a Goshawk. Subchronic air sac aspergillosis in a Goshawk.
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9.19 Deposition of uric acid crystals in the pericardium of a White Bellbird.
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9.22 Splenomegaly with necrotic foci in a Canary with subacute pseudo-tuberculosis.
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9.24 Liver with necrotic foci in Canary with subacute pseudo-tuberculosis.
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9.28 in a blood smear from a Buzzard. Hemacolor; original magnification X100.
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9.30 Mycotic processes (arrowed) in the lungs of a Capuchinbird with subacute aspergillosis.
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9.31 Gizzard with green koilin layer due to reflux of bile.
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9.32 Duodenum with pancreas (P).
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9.33 Candidiasis in a Gouldian finch.
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9.34 Trichomoniasis. Gross lesion in a Bengalese finch. in a crop smear from a pigeon.
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9.35 in a Wood Pigeon: proventriculus – serosal side. proventriculus – mucosal side; worm egg (50–58 × 22–29 μm) in faecal sample.
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9.38 PME report form and checklist.

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